Metastable transition studies로 peptides의 아미노산 배열을 추적하였다. 본 연구에서는 metastable transitions를 확인하기 위해 DADI/MIKE spectrometry를 적용하였다. pentapeptide인 enkephalin 유도체, oligopeptide들의 mixture 및 isomeric peptides mixture에 stepwise DADI/MIKE spectrometry를 적용한 결과 배열결정에 주요한 metastable ionpeaks를 얻을 수 있었다. 이러한 방법으로 얻어진 DADI/MIKE spectra로 용이하게 아미노산 배열을 결정할 수 있었다.
Sequence analysis of peptides was investigated by metastable transition studies. In the present work, metastable transitions of peptides were detected by direct analysis of daughter ions (DADI) or mass analyzed ion kinetic energy (MIKE) spectrometry. Stepwise DADI/MIKE spectrometry of enkephalin derivative, oligopeptides mixture, and isomeric peptides mixture were examined. Since prominent metastable ion peaks were observed, it was possible to elucidate peptide sequences from the DADI/MIKE spectra.
