Pipethanate*HCl reacts with ammonium cobaltothiocyanate to form a stable ion pair which has an absorption maximum at 626nm. The reaction product was insoluble in water but freely soluble in most organic solvents. 1, 2-Dichloroethane was the best extracting solvent among the several organic solvents. Pipethanate*HCl can be determined not only by the spectrophotometry but also inderectly by estimation of cobalt in the organic phase by atomic absorption spectrophotometry. Linear relationship was found between absorbance and concentration in the range of $1.0{ imes}10^{-3}~4.0{ imes}10^{-3}M$ by spectrophotometry and $1.5{ imes}10^{-4}~4.0{ imes}10^{-4}M$ by atomic absorption spectrophotometry. With this method it was possible to determine pipethanate$cdot$HCl in the pharmaceutical preparations.