Tobacco mosaic virus (TMV) was detected from Kimchi by biological and serological assay 5. Kimchi samples three month after cooked were collected, and were inoculated on N. tabacum var. Burley 21 and NC 95. Out of 33 samples, 6 showed typical symptoms induced by TMV, local necrotic lesions on Burley 21 and mosaic on NC 95. All saps from tobacco leaves showed the mosaic symptom reacted positively against TMV antiserum by agar gel double diffusion test. Based on the results, the Kimchi is considered as one of the important inoculum sources in Korea.