The study was concerned with the biological denitrification of wastewater using the fluidization of biofilm-coated carbon particles. And the effect of Glucose and biofilm thickness on denitrification was mainly investigated. Experimental results showed that biofilm thickness increased with the growth of bacteria and 14 days after the beginning of operation, the thickness approached 300-310${mu}{ extrm}{m}$. It was found that biofilm thickness was directly concerned with the removal efficiency of NO$_3$$^{[-10]}$ -N. As the results of experiments to find out the influence of Glucose on denitrification, Glucose, 60% excess from stoichiometric quantity was adequate and sufficient to react the bacteria with NO$_3$$^{[-10]}$ -N.