The purpose of this study was to examine the sealing ability of the vitapex, when used with gutta-percha cone, as a root canal filling material. Fourty five canals from extracted human maxillary and mandibular teeth were randomly selected and instrumented in a conventional method with k-file. After instrumentation and dry the canal with paper points, the canals were divided into three groups and fifteen canals in each group were filled with the following materials; Vitapex, Vitapex in combination with gutta-percha cone, and Gutta-percha cone and Zinc-oxide Eugenol Cement. All the specimens were immersed in 2% methylenblue dye solution and the depth of dye penetration into the canals were evaluated by macroscope at the intervals of 1 day, 2days and 7days. The following results were obtained; 1. All the materials experimented showed varying degrees of dye penetration. 2. The canals filled with Vitapex and Vitapex in combination with gutta perch a cone revealed sudden increase of dye penetration with time passage compared to the canals obturated with Gutta-percha cone and Zinc-oxide eugenol cement. 3. In the canals filled with Vitapex, the mean dye penetration was 1.6mm at 1day, but the specimen exposed to the dye for 7days showed mean dye penetration of 9.2mm. 4. In the canals obturated with Vitapex and gutta-percha cone, the mean dye penetration was 2mm at 1day, 2.2mm at 2days, and 8mm at 7days.