- 소의 Pepsinogen의 생합성
- Biosynthesis of Bovine Pepsinogen
- ㆍ 저자명
- 윤주억,김성기,Yoon. Joo-Ok,Kim. Sung-Kin
- ㆍ 간행물명
- 한국생화학회지
- ㆍ 권/호정보
- 1983년|16권 1호|pp.81-84 (4 pages)
- ㆍ 발행정보
- 생화학분자생물학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
소의 제4위점막의 total RNA로부터 oligo(dT)-cellulose 크로마토그라피와 Sepharose 4B 크로마토그라피로 poly(A) 함유 pepsinogen mRNA를 분리 정제하고, wheat germ cell-free system에서 pepsinogen translation반응을 실시하였다. Translation product는 sodium dodecyl sulfate-polyacrylamide gel 전기영동과 fluorgraphy로 분석한 결과 주성분이 분자량 45,000과 43,000 dalton의 두가지 다른 분자형의 pepsinogen임이 밝혀졌다.
Poly(A)-containing mRNA of pepsinogen was isolated from total RNA of bovine gastric mucosa by oligo (dT)-cellulose chromatography and Sepharose 4B chromatography, and was translated in a wheat germ cell-free system. The translation products were analyzed by $^4$sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. A major translation product was pepsinogen which was synthesized as two different molecular forms with molecular weights of 45,000 and 43,000 daltons.