한국의 토양에서 항생물질을 생성하는 균주를 분리하기 위하여, 토양으로 부터 분리한 균주의 항균력에 대한 Screening을 시행하였다. 그중 충청북도에서 채취한 토양에서 분리한 균주가 그람양성균에 매우 강한 항균력을 나타내어 이 균주에 대한 연구를 진행하였다. International Streptomyces Projeet methods 에 따라 생태학적 및 생리학적 성질을 관찰하여 Streptomyces에 속하는 종임을 확인하였다. 이 균주를 액내배양하여 얻은 항균성물질은 XAD-2와 CM-Sephades를 사용하여 정제하여 quinone류에 속하는 항생물질이라는 것을 밝혔다.
To isolate antibiotic-producing microorganisms from Korean soils, microbes were isolated from the soil samples and screened for antibacterial activity. A strain which was isolated from the soil sample collected in Choong Chung Book Do had a high antibacterial activity against gram-positive bacteria. The examination of morphological and physiological characteristics of that strain according to the International Streptomyces Project methods showed that it was one of Streptomyces species. After the antibacterial constituent of the strain was produced in submerged culture method, it was isolated and purified by XAD-2 and CM-Sephadex column chromatography. And it was found to be one of quinone type antibiotics.
