근원세포의 융합에 관여하는 융합유도물질의 존재를 구명하기 위하여 계배의 근세포를 배양하면서, muscle-conditioned medium (MCM) 이 근원세포의 융합에 미치는 영향을 조사하고, 근원세포로부터 배양액 내로 방출되는 단백질을 분석하여 다음과 같은 결과를 얻었다. (1) MCM은 뚜렷한 융합촉진 효과를 나타냈으며, 이러한 효과는 첨가된 MCM의 농도가 층가함에 따라 증가하였다. (2) MCM의 융합촉진 효과는 주로 근원세포로부터 방출되는 융합유도물질에 의하여 일어나는 것으로 판단된다. (3) 배양근원세포로부터 분비되는 45,000달톤과 65,000달톤의 단백질이 융합 유도물질일 가능성이 높다.
In order to investigate the mechanism of myoblast fusion during muscle differentiation in culture, the effect of muscle-conditioned medium on the fusion was studied and possible release from cultured myoblast cells of proteins which may be responsible for the promotion of myoblast fusion was analyzed. The muscle-conditioned medium showed a marked fusion-promoting activity in a dose-dependent fashion. THis fusion-promoting activity of the muscle-conditioned medium appeared to be due to the accumulation of at least two proteins which were released from the myoblast into the culture medium. These released proteins were analyzed by electrophoresis and autoradiography and found to have molecular weights of 45,000 and 65,000.
