인공수정으로 부화시킨 미꾸리자어에 대한 합성세제(LAS)의 급성독성 영향을 실험하고 그 결과를 생물검정법에 의하여 조사하였다. 1. LAS의 영향을 시간에 대하여 각 농도별로 보면 6ppm이 120시간, 18 ppm이 96시간, 30 ppm이 48시간, 그리고 38 ppm이 16시간만에 $100\%$의 폐사율 가져왔다. 2. 반수치사농도 ($LC_{50}$)는 48hr-$LC_{50}$이 12.59ppm, 96 hr-$LC_{50}$은 4.00 ppm, 120 hr-$LC_{50}$은 1.02 ppm이었다. 3. 급성독성에 대한 영향한계농도는 $0.37{sim}0.43ppm$이고 적용계수는 $0.093{sim}0.108$로 나타났다. 4. 합성세제의 각 농도에 대한 반수치사를 가져오는 시간($LT_{50}$)은 0.2 ppm이 165.1시간, 2ppm은 106.2시간으로 나타난 반면 8 ppm은 60.3시간으로 낮아져 38ppm의 경우는 23.5시간이었다.
Short-term acute toxicity of synthetic detergent(LAS) to larvae of loach, Misgurnus angillicaudatus was examined by static bioassay. The larvae were exposed to 15 different concentration of synthetic detergent for 16, 48, 72, 96 and 120 hours in order to determine median lethal concentration($LC_{50}$). The $100\%$ mortarlity of larvae was showed within 120, 96, 48 and 16 hours for 6, 18, 30 and 38 ppm, respectively. The median lethal concentration values of the larvae were 12.59 ppm for 48 hours, 4.00 ppm for 96 hours and 1.02 ppm for 120 hours. The permissible toxicant concentration of acute toxicity to larvae was $0.37{sim}0.43$ ppm, and application factor of the synthetec detergent was $0.093{sim}0.108$. The median lethal time($LT_{50}$) for different concentration also was determined. The $LT_{50}$ of 0.2 ppm was found within 165.1 hours and 2 ppm was 106.2 hours, while the $LT_{50}$ of 8 ppm was 60.3 hours and that of 38 ppm was 23.5 hours.
