This study was designed to evaluate the effects of tocopherol supplementation on serum $HDL_{2}$ and $HDL_{3}$ subfraction and lipoprotein pattern of young rabbits fed the different levels of dietary polyunsaturated fatty acids. White male rabbits with an average bo요 weight of 90kg were assigned into 4 groups : LP( Low PUFA) ; HP( High PUFA) ; LPT( Tocopheror added to LP), HPT(Tocopherol added to HP) by feeding safflower oil or coconut oil at loft( w/w )in addition to thew diet with or without tocopherol for 4 weeks. HDL-Chol and HDL subfractions were determined by polyanionic precipitation method, and the relative amount of lipoprotein by electrophoresis. Serum total cholesterol and TG wer slightly lowered in the group of high PUFA diet and its effect was more in the case of tocopherol supplemented. HDL-Chol level was reduced in the high PUFA diet with or without tocopherol, but overall, HDL-Chol level was not influenced by tocopherol addition and also no significant change in lipoprotein pattern when tocopherol added to the HP diet. $HDL_{2}$ level was increased in HPT group but not in LPT group when tocopherol was added to each HP and LP group, respectively. $HDL_{3}$ levels of both LP and HP groups were reduced with tocopherol supplement, but the reduction was less in the high PUFA groups so that $HDL_{2}$ : $HDL_{3}$ ratio of HP was rather close to that of LP, but it was increased when tocopherol supplemented to the HP diet. Therefore, high PUFA diet could improve the anti atherogenic factor when tocopherol supplemented.