In case of tissue characterization from ultrasonic echo signal, it is difficult to evaluate an absolute physical quantity because of redundancy in the echo signal. For it, in this paper a possibility of characterization of ultrasonic velocity distribution in multilayered medium using dip points analysis method from the reflected echo spectrum is discussed. To prove the possibility of this method, effect of interference phenomenon, impedance difference, attenuation, and thickness is discussed by computer simulation, and then the results confirm the effectiveness. And results of simple experiment are presented.