In the case of temperature control of air conditioning and refrigerating system, it is possible to operate the system continuously by controlling the cooling capacity of refrigerating machines. But on-off control system has been adopted for refrigerating system which has more large capacity than that required to remove the generated heat. In this on-off control system, it can be considered that there exists some optimum condition for the refrigerating capacity, operating cycle, running hour, and the temperature difference between thermostat setting value and real one. In this paper, an equation was derived to express the temperature variation of the refrigerated object (Nybrine) and later two evaluating functions were derived. One is for the temperature difference and another is for operating cycle and running hour. The weighted sum of these two functions is defined as the criterion function for the evaluation of the control performance of the system, and then the optimum running condition is investigated in the sense of minimizing the criterion function. Experiments showed that the heat balance equation derived for the temperature variation and the estimation of the time constant of the refrigerated system are appropriate. By conclusion, if a proper weighting factor D is selected, the optimum conditions exist for the refrigerating capacity, running hour, and operating cycle in the on-off temperature control of the refrigerating system.