알파파 근류로부터 DEAE-Cellulose (DE52) 및 Sephadex G-100 크로마토그라피에 의하여 5종의 leghemoglobin component를 분리 정제한 후 이것을 이용하여 antlserum을 만들었다. 한편 알팔파 근류에서 분리한 RNA로 부터 oligo (dT)-cellulose 크로마토그라피에 의하여 poly $(A)^+$-mRNA를 분리 정제하고 이들을 sucrose density gradient에 의하여 분획하여 in vitro translation시킨 후 면역 침전법에 의하여 leghemoglobin message 함유비가 높은 9S, 18S 및 28S mRNA를 정제하였는데 각 fraction의 leghemoglobin message 함유비는 각각 58%, 27% 및 36% 였다.
The five components of leghemoglobin from alfalfa root nodules were purified by DEAE-cellulose(DE52) and Sephadex G-100 chromatography and antiserum against the purified alfalfa leghemoglobin was prepared. RNA was extracted from alfalfa root nodules and poly(A)-mRNA was purified by oligo(dT)-cellulose affinity chromatography. The leghemolgobin messages were observed to be 23% of the poly(A)-mRNA. The poly(A)-mRNA was fractionated by sucrose density gradient centrifugation and the mRNA had 9, 18 and 28S value populations with 58, 21 and 36% of the leghemoglobin messages.
