The objective of tissue characterization is to provide quantitative information about the physical state of tissue interrogated by an ultrasound beam. In the computer simulation, it was found that the echoes were composed of the interferences of the ultrasonic waves reflected from both sides of the thin object, and could be separated by the spectral correlation method. Also, the phantom study demonstrates that thickness of the thin acryl layer beyond the resolution of common ultrasonic imaging systems can be measured- using this method.