This study was designed to observe the effect of temperature cycling on the production of aflatoxin by Aspergillus parasiticus R-716 in rice, barley, peanut and soybean. In those media, temperature cycling resulted in more total aflatoxin production by the strain of R-716 than constant incubation at $28{circ}$C and natural condition did. Especially, high level of total aflatoxin ($1826{mu}g$/30g) in rice medium at temperature cycling was produced. The intensity of yellow color of chloroform extracts correlated with the concentration of aflatoxin, and the ratio of aflatoxin $B_1$ to aflatoxin $B_2, G_1, G_2$ is lower at temperature cycling condition than at $28{circ}$C.