M. inyoensis NRRL 3292에 의한 시소마이신 생산에 영향을 미치는 인자들에 관해 조사한 결과 cobalt chloride 및 methionine 모두가 시소마이신 생산을 현저히 촉진하였으며, 탄소원으로서는 전분 또는 덱스트린이 포도당에 비해 시소마이신 생성에 적합하였다. 특히 시소마이신 발효공정중 항생물질 생성시기에 발효액내의 용존 이산화탄소 농도가 낮을수록 시소마이신 생성 농도가 증가하였다.
The effects of cobalt chloride, methionine, and various carbon sources on the sisomicin production by M. inyoensis NRRL 3292 were investigated. It was found that both cobalt chloride and methionine exerted a greater stimulatory effect on sisomicin formation. Kinetic studies with various carbon sources revealed thai polysaccharide such as starch or dextrin was found io be better than glucose for sisomicin production Moreover, the relatively low concentration of dissolved carbon dioxide was one of the most important factors In accelerating sisomicin production during idiophase.
