To determine relationships of supposed risk factors to positives for HBsAg and Anti-HBs and also relationships of subjective symptoms to positives for HBsAg and Anti-HBs, study of 658 people working in the hospital, university, bank and other office was performed. Positive rate for HBsAg was about 7.9% and positive rate for Anti-HBs was about 20.0%. Odds ratio of HBsAg was high and significant in individuals who are married and who have previous hepatitis B(P<0.001), medical personnel in family, more than 4 people in a room(0.01<P<0.05) Odds ratio of Anti-HBs was also significant in individuals who have previous hepatitis B, liver disease in family, more than 4 people in a room (0.01<p<0.05) No one was positive for HBsAg and odds ratio of Anti-HBs was significant in individuals who have previous HB vaccination. Transmission of HBV may be very closely related to familly aggregation factors.