10 IU L-lysine-$alpha$-oxidase와 젤라틴막 그리고 소형 산소전극으로부터 효소전극을 만들었다. 이의 반응은 효소막이 얇을수록 빨리 일어났으며 $120;{mu}m$의 효소전극에서는 1 mM까지 거의 직선적으로 증가하였다. 최대반응속도는 리신의 농도와 관계가 있어 최대 반응속도-리신농도로 표시한 표준곡선은 포물선으로 되었으며 $V_m$ app.는 6.0 mV/sec이었고 $K_m$ app.는 0.7 mM이었다. 이상의 방법으로 리신 농도를 연속흐름계에서 측정하였다. 최적유속은 3 cc/min이었으며 최대반응률은 반응후 12초 이내에 나타났다. 최적 효소농도는 10 IU이었고 최적 pH는 8.7이었다. 최적 이온강도는 0.05 mM이었으며 온도가 $1^{circ}C$ 증가함에 따라 약 3%의 반응오차를 가져왔다. 시료는 시간당 60회 이상을 측정할 수 있었으며 효소막 하나로 3,000회 이상 반복 측정할 수 있었다.
The enzyme electrode was fablicated by combining 10 IU L-lysine-$alpha$-oxidase, gelatine membrane and $pO_2$ micro electrode. The reaction was fast as the membrane was thin. It was increased the reaction linearly until 1 mM in $120;{mu}m$-membrane. The maximum reaction rate was correlated with L-lysine concentration. The standard curve was hyperbolic so as to $V_m$ app. being 6.0 mV/sec and $K_m$ app. being 0.7 mM L-lysine. L-lysine was measured with the continuous flow system. Optimal flow rate was 3 cc/min and the maximum reaction rate was appeared within 12 seconds after reaction. Optimal enzyme concentration was 10 IU, optimal pH was 8.7 and optimal ionic strength was 0.05 mM. It was about 3% reaction error for the temperature increase of $1^{circ}C$. Sample frequency up to 60/hour could be achieved. With one membrane, more than 3,000 determinations could be performed.
