고온성 cellulose 분해이용세균인 Herpetosiphon geysericola CUM 317 균주가 생성하는 ${alpha}-amylase$, ${eta}-amylase$ 및 glucoamylase를 황산암모늄 염석, DEAE-cellulose chromatography, CM-cellulose chromatography 방법으로 각각 부분정제하였다. 이들 ${alpha}-amylase$, ${eta}-amylase$ 및 glucoamylase의 감자 전분에 대한 Km치는 $2.31mg/m{ell}$, $7.69mg/m{ell}$ 및 $8.33mg/m{ell}$였으며, 각 분자량은 84,000 dalton, 76,000 dalton 및 80,000 dalton의 크기로 나타났다.
Extracellular ${alpha}-amylase$, ${eta}-amylase$ and glucoamylase produced by a thermophilic and cellulolytic bacterium, Herpetosiphon geysericola CUM 317, were partially purified by salting out with ammonium sulfate and by chromatography on a DEAE-cellulose column and on a CM-cellulose column. The Km values of ${alpha}-amylase$, ${eta}-amylase$ and glucoamylase for potato starch were $2.31mg/m{ell}$, $7.69mg/m{ell}$, and $8.33mg/m{ell}$. The molecular weights of ${alpha}-amylase$, ${eta}-amylase$ and glucoamylase were calculated to be about 84000 dalton, 76000 dalton and 80000 dalton, respectively.
