The genetic analysis was caried out on the boiling-off ratio of cocoon layer, and the amounts of sericin and fibroin in the F1 generation from the diallel crosses of silkworm. The result obtained were as follows : 1. Boiling-off ratio and the amounts of sericin adn fibroin in male and female were over dominqnce and partial dominance, respectively. 2. The additive effect of gene was higher then the dominance effect in the amounts of sericin and fibroin in male and female, and in boiling off ration in female. 3. The direction of dominance in the amounts of sericin and fibroin in male and female was positive and that in boiling off ratio negative. 4. The effect of three characters on the combining ability was significant and their general combining ability was higher than the specific combining ability.