The objective of this study is to review the basic theories related substrate removal in wastewater flow variations using submerged biofilm reactor. An aerated biofilm reactor is that in which influent organic substrates are aerobically oxidized by the microorganisms of biofilm grown on the surface of submerged media. No sludge is returned, and oxygen is supplied by diffusers. Three types of aerated biofilm reactor are one stage-central aeration, one stageup flow aeration and two stage-side aeration. The orders of substrate removal capacity in wastewater flow variations showed two stage-side aeration, one stage-upflow aeration and one stage-central aeration. The phenonmenon of nonclosing volid in upflow aeration type was superior to these in central-side aeration type. Attached biofilm masses in case of upflow, side and central aeration reactor were 1.0mg/cm$^2$, 4.1 mg/cm$^2$ and 0.93 mg/cm$^2$, respectively. Yield coefficient for biofilm was 0.31 to 0.48. Especially, removal efficiency can be increased remarkably according to the number of biofilm reactor and the packed condition of media.