우리나라 중부이남에서 생산되는 몇몇 천연물질들을 이용하여 Bacillus thuringiensis var. israelensis (B.t.i)의 포자생성과 spore-crystal복합체의 생물감수성을 조사하였다. 대두와 어분으로 조성된 배지에서 B.t.i를 배양했을 때 가장 높은 포자수와 생균수(6.5$ imes$$10^{11}$ spores/ml와 6.9$ imes$$10^{11}$ cells/ml)를 나타냈다. 대두와 어분으로 조성된 배지로부터 만든 spore-crystal복합체에 Culex pipiens유충과 Aedes togoi유충을 24시간 노출했을때 LD$_{50}$값은 각각 2.587$ imes$$10^{-2}$mg/L와 2.808$ imes$$10^{-2}$mg/L로 나타났다. B.t.i를 대두와 어분으로 조성된 배지에서 3$0^{circ}C$로 48시간동안 진탕배양(200 rmp)했을때 균주의 생장은 최대가 되었다.되었다.
Five media, formulated from some natural substances produced from South Korea, were assessed for growth, sporulation and insecticidal properties of B. thuringiensis var. israelensis. Also, bacterial powders, prepared from broth, were assayed against 3rd instar larvae of Culex pipiens and Aedes togoi. The highest number of viable cells and spores per milliliter (6.9${ imes}$10$^$11/ and 6.5${ imes}$10$^$11/) were obtained in medium containing soybean and fish meal. Powders from media containing soybean and fish meal were the most effective for the in sects with LD$sub$50,s/ of 2.587${ imes}$10$^$-2/ and 2.808${ imes}$10$^$-2/mg/L. for Cx. pipiens and Ae. togoi, respectively. The population density of the culture was at a maximum after 48 hours in incubation when it was grown in the media containing soybean and fish meal at 30$^{circ}C$ with rotary shaking.
