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젖소 수정란의 급속동결법 개발에 관한 연구
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  • 젖소 수정란의 급속동결법 개발에 관한 연구
  • Quick Freezing of Bovine Embryos
저자명
조충호,황우석,정창국,전윤성,이흥식,이창우,Jo. Chung-Ho,Hwang. Woo-Suk,Cheong. Chang-Kook,Jeon. Yun-Seong,Lee. Heung-Shik,Lee. Chang-Woo
간행물명
韓國臨床獸醫學會誌= Korean journal of veterinary clinical medicine
권/호정보
1987년|4권 2호|pp.449-455 (7 pages)
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한국임상수의학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Quick freezing of bovine embryos was attempted after they were predehydrated at room temperature. Combined solutions of 2M glycerol or 2M ethylene glycol in the presence of either 0.5 or 1.0M sucrose in phosphated buffered saline+20% calf serum were compared. The quick freezing method in which embryos were directly transferred in liquid nitrogen vapor for 2 minutes at - l70$^{circ}C$ before being plunged into liquid nitrogen was used. Post-thaw survival rates in 2M glycerol and 2M ethylene glycol were high with 0.5 M (55.6% and 53.3%) versus 1.0M(38.1% and 31.6%) sucrose(P < 0.05). But survival rates with 2M glycerol and 2M ethylene glycol were not significantly different. Transfer thawed embryos frozen with 2M glycerol and 2M ethylene glycol by 0.5M sucrose resulted in birthrates of 40.9% and 40.0%, respectively compared to 26.3% and 27.2%, respectively, for 1.0 M sucrose(p<0.05). This was 56.0% for fresh control.