The purpose of this study was to investigate the effect of occlusal splints on the masseteric silent period and the changes of the masseteric silent period after experimental bruxism with occlusal splints. In nine subjects, anterior occlusal splints were fabricated. The jaw-jerk reflex was induced by tapping over mandibular symphysis area with solenoid driven hammer and electromyogram of left masseter muscle was recorded. In the recorded electromyogram of left masseter muscle the silent period duration was measured. This procedure was done before insertion of anterior occlusal splints, after insertion of anterior occlusal splints, after 30 min experimental bruxism with anterior occlusal splints, and 3hr after removal of anterior occlusal splints. The result were as follows; 1. The mean silent period duration was increased after insertion of occlusal splints compared with the mean silent period duration before insertion of occlusal splints. 2. There was no change of the mean silent period duration after 30 min experimental bruxism with occlusal splints compared with the mean silent period duration after insertion of occlusal splints. 3. 3hr after the removal of occlusal splints, the mean silent period duration showed no difference from the mean silent period duration before insertion of occlusal splints.