To develop the comfortable pesticid-proof clothes, wearing trials for 7 types of existing pesticide-proof clothes were done measuring skin temperature, pulse rate, humidity and temperature of inside clothing and subjective feeling such as thermal, humid and comfort sensations at $28{pm}1^{circ}C$, $75{pm}5\%$ RH, 2.3 m/sec wind speed. (Experiment 1). On the base of above results, wearing trials of 6 types of new pesticide-proof clothes and 3 types of pesticide-proof clothes as control group were done with the same method as experiment 1. (Experiment 2). The results obtained were as follows 1. Heat stress was lower in newly designed pesticide-proof clothes with mesh running shirts. 2. For the body temperature regulation, it was confirmed that 6 types of new pesticide-proof clothes made of microporous fabric were better than the others.