The purpose of this study was to evaluate the sealing properties of endodontic cavity filling materials according to the time intervals after filling. Access cavities were prepared in extracted human premolar or molar teeth and filled with caviton, zinc oxide eugenol cement, zinc oxide eugenol cement with a base of gutta percha stopping and gutta percha stopping. After filling at the intervals of immediate, 2 days and 2 weeks the teeth were immersed for 2 weeks in 1% methylene blue solutions. Longitudinal sections were obtained from approximately center of teeth and the depth of dye penetration into the access cavities were observed by 10${ imes}$macrolens. The following results were obtained. I. All the materials experimented showed varying depth of dye penetration. 2. Of the material tested, caviton showed the best marginal sealing qualities regardless of the time intervals after filling and the sealing properties of the gutta percha stopping was the worst. 3. Both in zinc oxide eugenol cement and zinc oxide eugenol cement with a base of gutta percha stopping, the fillings allowed to mature for 2 days in normal saline solution showed the best sealing properties and those with no maturing time revealed the worst sealing qualities. 4. The sealing qualities of zinc oxide eugenol cement with a base of gutta percha stopping revealed slightly lower depth of dye penetration than that of zinc oxide eugenol cements.