The study was conducted to identify growth habit of Monochoria vaginalis gathered from three different locations (Suweon, Jeonju and Milyang). The plant height, number of leaves and dry weight of M. vaginalis gathered from three different locations were greatly affected by the seeding dates. The earlier the seeds of M. varginalis sown, the greater M. vaginalis grew, In general, the earlier seeding dates produced significantly longer plant height, number of leaves and dry weight than those of later seeding dates, showing no difference in the collection of weed seeds in different locality. The earlier seeding dates resulted in the earlier flowering, but the interval between dates of seeding and flowering decreased progressively as the seeding times were delayed. Seed production was not significant among three locations and but seeding date on June 15 produced th highest seed production as compared with other seeding times. No. of flowers and capsule per pot among yield components were two major factors affecting seed production.