Sprague Dawley 암쥐를 이유때부터 두군으로 나누어 각각 pyridoxine이 충분한 식이와 pyridoxine이 부족된 식이로 성장시킨 후, 적절한 시기에 임신시켜 태어난 새끼가 이유?瑛? 때 결핍식이군을 다시 두군으로 나누어 한군은 pyridoxine이 충분한 식이로 바꾸어 준 후 세군의 쥐를 35주까지 성장시켰다. 이 쥐들을 2, 3, 5, 7, 10, 35주에 임의로 몇마리씩 희생시켜서 뇌에서는 단백질, norepinephrine, dopamine, serotonin을 분석하였고 혈청에서는 GOT 활성을 측정하였다. 체중은 결핍군이 대조군보다 낮았으나 뇌의 무게는 큰차이가 없었다. 혈청 GOT 활성은 결핍군에서 대조군보다 유의적으로 낮았고 뇌의 단백질 함량도 대조군보다 결핍군에서 낮았으며 뇌의 norepinephrine, dopamine, serotonin 양은 대조군에 비하여 결핍군이 낮은 편이였다. 이 상으로써 장기간에 걸친 가벼운 정도의 pyridoxine 결핍은 새끼쥐의 뇌성장 발달에 영향을 미치는 것으로 생각되어진다.
Weanling female Sprague Dawley rats were fed diets containing 22 mg pyridoxine HCl/kg(control) and 1.2 mg pyridoxine HCl/kg(deficient). They were fed their diets throughout growth, gestation and lactation. After the pups were born and weaned, deficient group was divided into two groups. One(DC) switched to control diet and the other(DD) continued the same deficient diet until 35 weeks of age. Body and brain weights were measured on pups at 2,3,5,7,10 and 35 weeks old. At the same intervals, offsprings were randomly chosen and decapitated. Brain were analyzed for protein, norepinephrine, dopamine and serotonin. And serum GOT activity were determined. Body weight of deficient group was slightly lower than the control. But brain weight was not affected by pyridoxine depletion. Especially serum GOT activity of deficient group was significantly lower than the control and brain protein also depressed in deficient groups significantly at 2nd, 3rd and 7th weeks. Brain norepinephrine level of deficient group was slightly lower than the control and brain dopamine and serotonin level of deficient group were also slightly lower than the to control.
