야생형(野生型)인 만가닥버섯(Lyophyllum ulmarium ASI 8007)의 원형질체(原形質體)로부터 분리(分離)한 염색체(染色體)를 영양요구주(營養要求株)인 잔나비걸상버섯(Ganoderma applanatum ASI 7-18; cys met)의 원형질체내(原形質體內)에 $PEG+CaCl_2$로 섭입(攝入)하였다. 형질전환주(形質轉煥株)는 microtransgenome과 macrotransgenome type으로 전자(前者)는 느리고 불안정성(不安定性)인 균사생장(菌絲生長), 후자(後者)는 아주 빠른 안정성(安定性)인 균사생장(菌絲生長)이 있으며, 균사(菌絲)가 양친(兩親)보다 굵었으며 GCM+benomyl에서 균총분리(菌叢分離)가 일어났다. 이들을 전기영동으로 esterase 동위효소(同位酵素) 패턴을 조사한 결과 양친에 비하여 위치가 다르게 나타났다.
The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen $glycol+CaCl_2+glycine$. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.
