간장덧에 분리 동정한 Zygosaccharomyces rouxii M-12 와 Saccharomyces cerevisiae M-43간의 원형질체 융합에 의한 새로운 간장효모균주를 개량할 목적로 영양요구성 돌연변이주인 Zygosaccharomyces rouxii ZRM-83 ($Met^-,;Thr^-$)과 Saccharomyces cerevisiae SCM-465($Lys^-,;Arg^-$)을 얻었다. 양 균주의 원형질체 형성의 조건은 Zymolase 20T을 0.5mg/ml, 60분에서 많이 생성하였고 원형질체 융합조건은 30% PEG6,000을 30분간 처리했을때 융합빈도는 $10^{-4}{sim}10^{-5}$이였다. 융합체의 세포크기는 진주보다 컸으며 DNA함량도 많았다.
Protoplast susion between Zygosaccharmoyces rouxii M-12 and Saccharimyces cerevusuare M-43 were investigated for breeding of a new brewing yeat strain for soy sauce. Auxotrophic mutants of Zygosaccharomyces rouxii ZRM-83 ($Met^-,;Thr^-$) and Saccharomyces cerevisiae SCM-46 ($Lys^-,;Arg^-$) were selected by treatment of 3.0% ethylmethane sulfonate and nutritional complementary method. Protoplast of both strains were more effective by treatment of 0.05mg/ml zymolase 20T for 60min. Fusion effeciency was much higher by treatment of 30% PEG 6,000 for 30min and fusion frequencies were $10^{-4}{sim}10^{-5}$. These fusants originated from two protoplasts had properties of big cell size and much DNA content.
