- 육우수정란 간역동결 및 융해방법에 관한 연구 제육보. 내동제에 Sucrose 첨가에 따른 액체질소에 미치는 영향
- ㆍ 저자명
- 이중계,이규훈,강만종,김영훈,문성호,김승호
- ㆍ 간행물명
- 가축번식학회지
- ㆍ 권/호정보
- 1988년|12권 2호|pp.77-83 (7 pages)
- ㆍ 발행정보
- 한국동물번식학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
This study was done with mouse embryo to assess effects of freezing media containing sucrose, freezing metods(1-F, 0.3$^{circ}C$/min;2-F, 3-5$^{circ}C$/min;3-F, 15$^{circ}C$min;4-F, LN2 vapour) and cell freezers on the embryo survival determined using the FDA test. The results are summarized as follows. 1. The FDA score obtained with 1, 2, 3 and 4-F was 3.8, 3.6, 3.2 and 3.2, respectively. There was a significant difference(P<0.05) between 1-F, 3-F and 2-F, 4-F. 2. The score at the morular stage(3.8) higher(P<0.005) than the blastocyst stage of embryos(3.2). 3. No difference (P>0.05) was found between the score obtained with a automatic embryo freezer(4.0) and a liquid nitrogen container(3.7).