This study is an experimental research on the anaerobic digestion of pig manure and night soil mixed waste at room temperature (25$circ$C), and the results are shown below: 1. The steady-state condition based on gas production as digestion temperature dropped to 25$circ$C from 35$circ$C was achieved at around 28, 47, 56, 64 days respectively when its hydraulic retention time(HRT) are 10, 20, 30, 40 days. 2, Alkalinity and volatile acid(VA) was increased as increasing the organic loading. 3. Removal efficiency of chemical oxygen demand(COD) and biochemical oxygen demand (BOD) was improved as longer HRT, and generally COD value is lower and BOD value is higher relatively. 4. Overall treatment efficiencies of mixed waste are higher than of pig manure and of night soft. 5. Organic removal efficiency at room temperature (25$circ$C) is 20-25% lower at medium temperature (35$circ$C) in a same VS loading condition. 6. Refractory fraction of the infiuent VS and organic removal rate constant(K) estimated at around 37% and 0.107/day respectively.