Both Cobitis lutheri Rendahl and C.striata Ikeda previously regarded as the subspecies of C.taenia are revised here and raised to the species rank based on the distinct color pattern on their body sides in relation to the shpae of lamina circularis and suborbital spine, and distinct distributional patter. C. lutheri was similar to C. striata in chromosome number and karyotype, but chromosomal polymorphism as Robert sonian event was confirmed only in the population of C.lutheri studies. Both, C. kutheri and C..striata have disjunct ranges : the former in western Korea and east-northern China Mainland, the latter in the Smjin River of korea and west-southern Japan. hybridization between C. lutheri and C. striata by secondary contact appeared in the limited zone of the Dongjin River, Chllabuk-do province, korea, but the evidence for habitat segregation between them suggests the possibility that natural hybridization occurs between the two species and introgression results. We consider that the two species were produced as ecological equivalent species in the different branch stream of the Paleo-Hwangho River , The time of recession of sea level during the gracial period.