밤바구미 (Curculio dentipes Roelofs)의 발생 단계에 따른 유약호르몬과 유약호로몬 특이 에스테라제의 활성도를 비교 측정한 결과는 다음과 같다. 1. 단백질 함량은 비월동유충에서 최고의 함량을 나타냈으며 월동중에 감소하다가 월동 후 다시 증가하였으나 종령유충기부터 계속 감소하여 용시기에 최저 함량을 나타내었다. 2. 유약호르몬 활성은 비월동 유충에서 가장 높게 나타난 반면 종령유충 이후 용시기에 이르면서 그 활성이 아주 낮아졌다. 3. 유약호르몬 특이 에스테라제의 피이크는 월동중 유충기가 종령유충기에서 나타났다. 4. 유약호르몬의 활성과 유약호르몬 특이 에스테라제 활성과의 관계는 서로 역비례 관계를 나타내었으나, 단백질 함량과 유약호르몬 특이 에스테라제의 활성관계는 같은 양상으로 나타났다.
The concentration of total protein, juvenile hormone (JH) and juvenile hormone esterase (JHE) were measured in homogenates of chestnut weevil, Curculio dentipes Roelofs according to developmental stages, respectively JHE levels were monitored using juvenile hormone I as a substrate while juvenile hormone titers were measured with Galleria bioassay. The results obtained are as follows: The protein contents were found to be the maximum at the non-overwintering larvae and decreased at the wintering larvae. At the overwintering, they increased again but reduced at final instar larvae and then reached the minimum level at the pupae. The juvenile homone titer showed the highest activity at non-overwintering larvae whereas it reached the lowest level at final instar larval and pupal stages. The activity peaks of the juvenile hormone specific esterase in homogenates were shown at wintering larvae and final instar larval stage. The activity of juvenile hormone specific esterase per mg of total protein was lowest at non-overwintering larvae and it increased at wintering larvae but reduced at overwintering larvae. At last, it reached the maximum at pupal stage. The relationship between juvenile hormone titer and juvenile hormone specific esterase was shown an inverse proportion and also the relationship between total protein and juvenile hormone specific esterase was the same aspect.
