If proper design and selection of the working fluid are made the power generation system of temperature difference could achieve more efficient results than others. This paper is to analysis the production of its power generation due to several parameters. Making the power generation system, the characteristics of power output are investigated to obtain its basic data for design. This results of this experiment are as follows. 1. The most proper working fluid in the system is Freon-22 having high stability and difference between the outlet pressure, $P_E$ of evaporator and outlet pressure, $P_c$ of Condenser. 2. With the increase of temperature difference between evaporator and condenser, the output in the system increases linearly. 3. The generation efficiency is largely dependent on the type or form of propeller, nozzle and optimum design of heat exchanger.