연제품의 보존제로서 lysozyme의 효과를 검토하기 위하여, 부패된 연제품에서 7종의 대표 균주를 분리 선정하여 lysozyme에 의한 발육조지 효과와 hexametaphosphate와 pyrophosphate을 병용 첨가하였을 때의 발육조지 효과를 측정하고 각 균에 대한 항균제의 발육조지 농도를 측정한 후 가장 효과적인 농도를 게맛살, 어묵과 튀김어묵에 첨가 또는 침지하여 $30^{circ}C$에 7일간 저장하면서 생균수, pH 및 VBN을 검사한 결과는 다음과 같다. 1. 분리 세균 7종 중 6종의 균은 lysozyme에 의하열 발육이 억제되었으며, sodium hexametaphosphate와 sodium pyrophosphate를 병용 첨가하였을 때 그 발육조지 효과가 상승하였다. 발육조지 효과는 lysozyme + sodium hexametaphosphate + sodium pyrophosphate, lysozyme + sodium pyrophosphate, lysozyme + sodium hexametaphosphate, lysozyme의 순으로 높았으며 저항력이 제일 강하였던 1균주도 lysozyme$(0.05\%)$ + sodium pyrophosphate$(0.5\%)$ + sodium hexametaphosphate$(0.1\%)$를 첨가하였을 때 발육조지 효과가 비교적 좋았다.2. 게맛갈과 어묵은 lysozyme$(0.05\%)$ 단독 첨가보다 sodium pyrophosphate$(0.5)\%$와 sodium hexametaphosphate$(0.1\%)$를 병용 첨가하였을 때 세균발육 억제효과가 상승하였으나 직접 균의 배양 시험에서 보다 효과가 적었다. 튀김어묵을 lysozyme$(0.05\%)$ 단독 그리고, lysozyme$(0.05\%)$ + sodium pyrophosphate $(0.5\%)$ + sodium hexametaphosphate$(0.1\%)$ 용액에 5분간 침지 처리하였을 때 약간의 방부효과는 인정되었으나 sorbic acid$(0.2\%)$을 첨가한 것보다 효과가 적었다.
Inhibitory effects on bacterial growth by using lysozyme and mixtures of it with other antibacterial substances (sodium hexametaphosphate and sodium pyrophosphate) were investigated against the 7 kinds of hacterial strains isolated from putrefied surumi products. The growth inhibitory concentrations of lysozyme and lysozyme + sodium hexametaphosphate + sodium pyrophosphate against the bacteria were added to kamaboko, imitation crab meat and fried surumi, then viable cell count, pH and VBN were examined during the storage at $30^{circ}C$ for 7 days. Lysozyme showed growth inhibitions against 6 of 7 isolates and the inhibition effect of mixture of antibacterial substances was multiplied against all the isolates compare with those of its individual use. Growth inhibitory effect of the substances on the bacteria was high in order of lysozyme + sodium pyrophosphate + sodium hexametaphosphate, lysozyme + sodium hexametaphosphate, lysozyme + sodium pyrophosphate and lysozyme. The most effective inhibitory concentration of mixture of the antibacterial substances in kamaboko and imitation crab meat was $0.05\%$ of lysozyme, $0.5\%$ of sodium pyrophosphate and $0.1\%$ sodium hexametaphosphate. But the bacterial growth was slightly inhibited in fried surumi even if the same concentration of the dipped mixture and the effect of the mixture was less than that of $0.2\%$ sorbic acid.
