The purpose of this study was to evaluate the effect of canal filling methods and post-space preparation time on the apical seal of endodontically treated teeth. Seventy-six single-rooted human teeth were divided into 6 groups and each tooth was obturated and prepared post-space according to the purpose of this study. After all specimens were immersed in Indian ink, decalcified and cleared, the degree of dye penetration into the root canals observed by magnifying glass (X20) and measured by caliper. The results were as follows: 1. In the thermatic condensation, no significant difference in ink penetration occured when the post-spaces were created immediately after obturation or when they were made a week later. 2. In the lateral condensation, no significant difference in ink penetration existed between teeth whose post-space were prepared immediately or delayed. 3. In comparison of experimental groups and control groups, no significant difference showed when the groups were obturated with McSpadden compactor but when the teeth were laterally condensed, the experimental groups showed less ink penetration than controls. 4. Thermatic condensed teeth with McSpadden compactor were less ink penetration thn laterally condensed teeth.