Retrograde ejaculation, an infrequent cause of male infertility, may be the sequala of prostate or bladder neck surgery or the result of interruption in the sympathetic innervation, the diagnosis is established by history and examination of urine. Infertile couple artificial insemination homologous(AIH) using retrograde ejaculate recovered from bladder has been successfully acomplished. In this case, ovulation was induced by clomiphene citrate, osmorality and pH of urine was controlled by buffer solution and immediately specimen collection, to improve sperm mobility. We had experienced a successful pregnancy and delivery case by above method. So here reported with brief review of literature.