고상법으로 개량된 반응용기를 이용하여 angiotensin II 유사물인 ($Ala^1$), ($Ala^2$) 및 ($Ala^3$) angiotensin II를 합성하였으며 그 수득률은 62.2에서 68.0%이었다. Pressor activity는 alanine이 N- 말단에 가까울수록 감사하였으며 그 최대치는 80.5%이었다. 이들 펩티드의 정제 및 순도결정은 gel filtraction, paper chromatography, thin layer chromatography, 전기영동, 녹는점 측정 및 아마노산 분석기에 의거하였다.
Synthesis of ($Ala^1$), ($Ala^2$) and ($Ala^3$) analopgues of angiotensin II by solid phase method using an improved reaction vessel was carried out. The yield average 62.2-68.0%. Homogeneity and purity of the three analogues synthesized peptides were established with paper chromatography, paper electrophoresis, amino acid analysis and melting point apparatus. The pressor activity was gradually increased with the increase in the chain length from the N-terminal to Ala residue angiotensin II, however the maximum value was within 80.5%.
