새로운 조각매물고둥렉틴인 NIC는 황화암모늄침전, 이온교환크로마토그래피 및 친화성크로마토그래피의 방법으로 분리, 정제되었다. 이는 전기영동상에서 한개의 주된 띠와 세개의 희미한 띠를 나타냈으며, specific activity는 82.16에서 85333.33 units/mg으로 증가되었다. Sephadex G-200 크로마토그래피와 SDS 전기영동결과, NIC는 분자량이 약 112,000으로 나타냈으며, 이는 분자량 약 14,000인 subunit의 octamer로 추정되었다. NIC는 2.5%의 당을 함유하는 당단백질이었으며, lactose, galactose 및 N-acetyl-D-galactosamine과 특이하게 결합하였다. 이의 아미노산조성은 산성아미노산의 함량이 많았으며, sulfur를 함유한 아마노산의 함량은 적었다.
NIC, another Neptunea intersculpta lectin, was purified by salt fractionation, DEAE-cellulose 52 ion exchange chromatography and lactosyl-Sepharose 4B affinity chromatography. Through these purification procedures, specific activity of NIC was increased from 82.16 to 85333.33 units/mg. And on polyacrylamide gel electrophoresis, NIC exhibited one major and three minor bands. A molecular weight of NIC was calculated as 112,000 by Sephadex G-200 gel filtration and the subunit was determined as Mr 14,000 by sodium dodesyl sulfate polyacrylamide disc gel electrophoresis. Consequently, NIC is supposed to be an octamer composed of identical subunits of approximately Mr 14,000. NIC was a glycoprotein containing 2.5% of neutral sugar and had specificity for lactose, galactose and N-acetyl-D-galactosamine. NIC contained relatively high amounts of acidic amino acids, but the contents of sulfur containing amoino acids was very low.
