Binding parameters of some edible dyes with bovine serum albumin were determined at pH 7.4 buffer solution. The edible dyes used were fast green, brilliant blue FCF, indigo carmine and allura red AC, and the final concentrations of each dyes were $3{sim}9{ imes}10^{-6}M$, $6{ imes}10^{-6}M$, $3{ imes}10^{-5}M$ and $3{ imes}10^{-5}M$, respectively. The final concentrations of bovine serum albumin were $1{sim}7{ imes}10^{-5}M$. The values of binding free energy between edible dye and protein were ranged from -6,024 to -6,800 cal/mole.