This study was carried out to investigate the effect of LH-RH and Gn-RH treatment in Holstein cows with ovarian quiescence and follicular cystic ovaries. The cows with ovarian quiescence and follicular cystic ovaries injected intramuscularly with 100$mu extrm{g}$, 200$mu extrm{g}$ and 400$mu extrm{g}$ of LH-RH and 200$mu extrm{g}$ and 400$mu extrm{g}$ of Gn-RH respectively. The cows was diagnosed by repeated rectal palpation. The plasma progesterone and estradiol-17$eta$ concentrations were assayed by radioimmunoassay methods. The resutls of this study were summarized as follows : 1. Ovulations were induced after treatment of LH-RH and Gn-RH. The concentrations of progesterone reached small peak level at luteal phase and estradiol-17$eta$ reached obvious peak level with the development and maturation of the follicle during the periods of degeneration of the corpus luteum, and normal ovarian cycle activity started subsequently. 2. The cows with ovarian quiescence and follicular cystic ovaries were induced ovulation at 38.9$pm$5.3 hrs. after treatment of LH-RH in 66.7% cows and at 52.7$pm$7.9 hrs after treatment of Gn-RH in 60.0% cows respectively. 3. The good ovarian responses were indicated in treatment with 200$mu extrm{g}$ to 400$mu extrm{g}$ of LH-RH than those treated with 100$mu extrm{g}$ in cows with ovarian quiescence, and did not show difference of ovarian responses between treatments with 200$mu extrm{g}$ to 400$mu extrm{g}$ of Gn-RH in cows with follicular cystic ovaries.