A study was made of the puff-by-puff delivery of menthol and tar for several commercial brands. The purpose of this study is for the brands constantly to maintain fresh aroma of menthol till the last puff in smoking. The results from this study could be used for development and evaluation of a mentholated cigarette. Prior to the purpose, a straightforward method from extraction through quantitative analysis of 9-menthol contained in cigarettes has been developed.