This experiment was carried out to investigate the effects of the first harvesting times and regrowth periods after the fist harvest on grass regrowth, dry matter (DM) yield and botanical composition in pasture mixtures during 1987 and 1988. For the test, a split plot design with 3 replications was treated with 3 different first harvesting times (vegetative, stem elongation and heading stage) and 3 different regrowth periods (20, 30 and 40 days) after first harvest. Although the total DM yield of grasses was decreased slightly with earlier cutting, the regrowth DM yield and the regrowth plant height was increased significantly with earlier cutting (p < 0.05). Also, the 30 days of regrowth periods was contributed greatly to the regrowth and the DM yield. The percentage of legumes Tmainly red clover) in botanical composition was increased with later initial harvest anrl longer regrowth period after first harvest. Based on the results, it is suggested that good grass regrowth and seasonal distribution could be achieved by the earlier harvest at fist and the regrowth period of 30 days after first harvest in spring.