- Xylose 발효효모의 Xylanase 생성
- Nutritional Conditions of Xylanase Production from Xylose Fermenting Yeast
- ㆍ 저자명
- 배명애,김남순,방병호,서정훈
- ㆍ 간행물명
- 산업미생물학회지
- ㆍ 권/호정보
- 1989년|17권 2호|pp.85-87 (3 pages)
- ㆍ 발행정보
- 한국미생물생명공학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
토양으로부터 분리한 Xylose 발효효모 X-6-41 (Candida sp.)의 xylanase 효소생성 조건을 조사해 본 결과, 본 실험에 사용한 X-6-41 균주는 배지조성중 yeast extract 혹은 polypeptone이 첨가되면 xylanase 효소생성이 없었다. 즉 특정 아미노산인 isoleucine에 의해서는 그 생성이 억제되었고, tryptophan 25$mu$g/$mell$ 농도의 존재하에서는 xylanase 생성이 증가했다.
Cultural conditions for the formation of extracellular xylanase by Candida sp. X-6-41 were investigated. The xylanase was not produced in culture medium containing polypeptone or yeast extract as a nitrogen source, respectively, whereas the enzyme w8s produced in chemically defined medium containing (NH$_4$)$_2$SO$_4$as a sole nitrogen source. The xylanase production was affected by the amino acids such as isoleucine and tryptophan. The enzyme production of the strain was completely inhibited by the addition of isoleucine in the culture medium, but enhanced by tryptophan below the concentration of 25$mu$g/$mell$.