Pseudomonas delafieldii이 다당류 생산을 위한 배양조건을 검토하였다. 최적 배지조성은 glucose 25g/$ell$, peptone 2.06 g/$ell$, yeast extract 0.5g/$ell$, KH$_2$PO$_4$2g/$ell$, MgSO$_4$2g/$ell$, CaCO$_3$2.5g/$ell$이며 교반속도는 300rpm, 온도는 30도, pH는 6.5이었다. 이 조건에서는 flask culture를 할 경우 5.91g/$ell$가 생산되며 이 때 수율은 23.65%, overall productivity는 50.98mg/g/h이었다.
The cultural condition for polysaccharide production by Pseudomonus detafietdii was studied. The optimal medium contains the following composition per liter of distilled water: glucose (25g/$ell$), peptone (2.06g/$ell$), KH$_2$PO$_4$(2g/$ell$), MgSO$_4$.7$H_2O$ (2g/$ell$), yeast extract (0.5g/$ell$), CaCO$_3$(2.5g/$ell$). The temperature and pH optimum were 3$0^{circ}C$ and 6.5. The agitation speed was 300 rpm. 5.91g of polysaccharide was produced at the condition in flask culture.
