The purpose of this study was to investigate the influence of balancing interference on the activity of masticatory muscles and condylar path. Eight dental students of Won Kwang University without any symptoms of temporomandibular disorder and occlusal interferences, were selected for this study, The balancing interference was provided by construction and cementation of cast metal crowns on the upper and lower first molars. For the measurement of muscle activity, bioelectric processor (EM2, Myotronic Res., Inc., U.S.A.) was used and for the condular path, computerized electronic pantograph (Pantronics, Denar Corp., U.S.A.) was used and the myographic recordings were taken bilaterally from the anterior temporal, masseter and digastric muscles on rest position and on functions. These experimental procedures were done before cementation of experimental crown, three days after cementation of experimental crown, one week after, two weeks after and then one week after removal of experimental crown. The results are as follows: 1. The PRI score was increased at three days after application of balancing interference, and decreased at two weeks after. 2. Three subjects showed mild symptom of temporomandibular disorder at three or four days after application of interference, but the symptom was subsided in one or two weeks after application of interference. 3. One week after application of balancing interference, the activity of ipsilateral anterior temporal muscle in four subjects was decreased on gum chewing at experimental site. 4. Three days after application of balancing interference, the activity of ipsilateral anterior temporal muscle in three subjects was increased on gum chewing at non-experimental site. 5. The influence of balancing interference on the activity of anterior temporal, masseter and gigastric muscle was not prominent.