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Vitrification에 의한 동결보존이 토끼수정란의 생존성에 미치는 영향
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  • Vitrification에 의한 동결보존이 토끼수정란의 생존성에 미치는 영향
저자명
김희석,양보석,오성종,이근상
간행물명
가축번식학회지
권/호정보
1990년|14권 1호|pp.43-49 (7 pages)
발행정보
한국동물번식학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

To improve the freezing techniques of animal embryos using vitrification solution as a cryoprotectant rabbit embryos, by cell stages, dehydration temperature and dehydration temperature and dehydratin time, were frozen-thawed and cultured. Following are the main results obtained. 1. The damage rate of zona pellucida after thawing was higher(13.6%) when the cell stage of embryos was less than 4 cells than when the cell stage was 8~16 cell or morula. The damage rate was higher when the dehydration temperature was 4$^{circ}C$ than -3$0^{circ}C$ or -50~-8$0^{circ}C$. The zona pellucida was damaged more when dehydrated for 5 min than when dehydrated for 10~15 min. 2. After being cultured for 72 hours, 5.3% of 4 cell(or less) embryos were developed to morula, while 86.4% of morula embryos were developed further. 3. More percentage of embryos(73.2%) was developed when dehydrated at -3$0^{circ}C$ than when dehydrated at 4$^{circ}C$ at -5$0^{circ}C$~-8$0^{circ}C$. 4. The hatching rate was higher when dehydrated for 5 min. When the embryos were dehydrated for 10~15 min and cultured for 24 hours, they were not even developed or development was not good in later stages.