- Vitrification에 의한 동결보존이 토끼수정란의 생존성에 미치는 영향
- ㆍ 저자명
- 김희석,양보석,오성종,이근상
- ㆍ 간행물명
- 가축번식학회지
- ㆍ 권/호정보
- 1990년|14권 1호|pp.43-49 (7 pages)
- ㆍ 발행정보
- 한국동물번식학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
To improve the freezing techniques of animal embryos using vitrification solution as a cryoprotectant rabbit embryos, by cell stages, dehydration temperature and dehydration temperature and dehydratin time, were frozen-thawed and cultured. Following are the main results obtained. 1. The damage rate of zona pellucida after thawing was higher(13.6%) when the cell stage of embryos was less than 4 cells than when the cell stage was 8~16 cell or morula. The damage rate was higher when the dehydration temperature was 4$^{circ}C$ than -3$0^{circ}C$ or -50~-8$0^{circ}C$. The zona pellucida was damaged more when dehydrated for 5 min than when dehydrated for 10~15 min. 2. After being cultured for 72 hours, 5.3% of 4 cell(or less) embryos were developed to morula, while 86.4% of morula embryos were developed further. 3. More percentage of embryos(73.2%) was developed when dehydrated at -3$0^{circ}C$ than when dehydrated at 4$^{circ}C$ at -5$0^{circ}C$~-8$0^{circ}C$. 4. The hatching rate was higher when dehydrated for 5 min. When the embryos were dehydrated for 10~15 min and cultured for 24 hours, they were not even developed or development was not good in later stages.