Serratia marcescens를 $30^{circ}C$에서 진탕배양했더니, 적색색소인 prodigiosin이 초로기(senescent phase of growth)에서 생성되었다. 그리고 이조건에서 배양한 세포를 polystyrene dish를 사용하여 세포의 hydrophobicity를 측정한 결과 상당한 소수성 성질이 발현되어 대부분의 세포가 비극성 성질의 polystyrene dish에 흡착되었다. 그러나 이 박테리아를 $37^{circ}C$에서 배양했더니, 적색 색소인 prodigiosin도 생성되지 않았을 뿐 아니라 소수성 성질도 발현되지 않음으로서 세포가 polysyrene dish에 흡착되지 않고 pre-washing 단계에서 모두 씻겨져 났다. 또한 $30^{circ}C$와 $37^{circ}C$에서 배양한 serratia marecescens의 지질성분을 분석한 결과, $30^{circ}C$에서 배양한 세포의 지질은 phospholipid, glycolipid 및 확인되지 않은 지질 등이 생성되었으나 $37^{circ}C$에서 배양한 세포의 경우는 주로 양쪽성 성질의 aminolipid인 serratamolide가 생성되어, 배양한 온도조건에 따라 뚜렷한 차이를 보였다.
S. marcescens cultured at $30^{circ}C$ with vigorous shaking was shown to produce red-pigment, prodigiosin, in the senescent phase of growth. Also, it showed many hydrophobic characteristrics, which were tested by the adherence to noncharged surfaces of polystyrene dishes, a typical agent for the binding of hydrophobic cells and molecules. However, when the cell was cultured at $37^{circ}C$, it no longer produced either red pigment or hydrophobic materials. Therefore, the bacteria cultured at $37^{circ}C$ was completely washed-out from the polystyrene dishes at the copious washing step with tap water, in contrast to the cell cultured at $30^{circ}C$ which was sticked onto the polystyrene dishes very tightly. The lipid compositions extracted from the S. marcescens cultured at $30^{circ}C$ or $37^{circ}C$ were very different from each other; the phospholipids, glycolipids and unidentified lipids were produced from the cell cultured at $30^{circ}C$, whereas large amounts of serratamolide, amphipathic compound, were produced from the cell cultured at $37^{circ}C$. The data suggest that the pronounced cell surface hydrophobicity of the S. marcescens is mediated by a combination of several surface factors that were affected by cultivation time and temperatures.
