담배거세미나방 핵다각체병 바이러스(Spodoptera litura nuclear polyhedroses virus: SINPV) 미생물살충제로 개발하기 위하여 기주식물, 온도 보관조건 및 태양광선에 의한 병원성 및 안정성에 대하여 조사하였다. 콩잎에 살포한 SlNPV의 담배거세미나방 3령과 5령 유충에 대한 $LC_{50}$은 각각 $1.301 imes10^{4 PIBS}/ml$와 $1.087 imes10^{5 PIBS}/ml$였으며 $1.0 imes10^{6 PIBS}/ml$에 대한 $LC_{50}$은 3령과 5령에 대하여 각각 7.3일파 8.9일이었다. SINPV는 $60^{circ}C$에서 4시간, $60^{circ}C$에서 1 10분 처리하였을 때 활성이 저하되었으며 처리시간이 길어질수록 급격한 활성감소현상을 보였다. 또한 SINPV는 $-20^{circ}C$ 동결보관이 $4^{circ}C$나 $25^{circ}C$ 보관보다 안정적인 활성을 유지하였다. 콩잎표변에 살포한 SINPV는 콩잎표변에 살포한 경우 3일 후에 불활화하였으나 콩잎 이면살포에서는 10일간 활성이 지속되었다.
A nuclear polyhedrosis virus (NPV) of the tobacco cutworm, Spodoptera litura would be a promisible agent for the control of the insect. To develop a viral insecticide using S. litura NPV, effect of spray on soybean leaves, temperature, storage, an sunlight on the pathogenicity of the virus were studies as follows: Median lethal concentration ($LC_{50}$) of the virus sprayed on the leaves against the third and the fifth instar larvae were $1.301 imes10^{4 PIBS}/ml$ and $1.087 imes10^{5 PIBS}/ml$, respectively. On the concentration of $1.0 imes10^{5 PIBS}/ml$, median lethal times ($LT_{50}$) were 7.3 days for the 3rd and 8.9 days for the 5th instar larvae. Stability of S. litura NPV was quickly decreased at the higher temperate than $60^{circ}C$ and at the longer exposure to the higher temperature. Storage of the virus at $-20^{circ}C$ was kept higher pathogenicity than $4^{circ}C$ and $25^{circ}C$. Viral activity was maintained more than 10 days in the sprayed-under leaves, but decreased at 3 day after spray in th sprayed-on the leaf surface when exposed the virus to sunlight.
